Determination of tocopherols content in sunflower oil during oxidation using fluorescence technique
The fluorescence intensity of tocopherols originated from cold-pressed sunflower oil at different degree of oxidation in presence of 1,2-dipalmitylo-sn-glicero-3-
-fosfatidylocholine lipid (DPPC) membrane have been measured. It has been shown that fluorescence of tocopherol depends on amount of oil as well as on membrane concentra-tion in the sample. Addition of oil with increasing peroxide value PV decreased the toco-pherol fluorescence due to its disappearance in the sample. At constant membrane con-centration chromatographically determined the amount of tocopherol in oil sample corre-lated with peroxide value and fluorescence intensity. Such results allowed us construct calibration curve which may be used for fast and accurate peroxide value determination using fluorescence technique.
sunflower oil, tocopherols, peroxide value, DPPC membrane, fluorescence
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Paweł
Górnaś
Aleksander
Sieger
Krzysztof
Dwiecki
Małgorzata
Nogala-Kałucka
Krzysztof
Palewski