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Issue 11 (1) 2012 pp. 83-89

Dorota Martysiak-Żurowska, Weronika Wenta

Department of Food Chemistry, Technology and Biotechnology, Gdańsk University of Technology, Poland

A comparison of abts and dpph methods for assessing the total antioxidant capacity of human milk

Abstract

Introduction. The Total Antioxidant Capacity (TAC) of human milk reflects the concentration and the activity of many components which prevent oxidative degradation of fats and proteins. This study compares the effectiveness of ABTS and DPPH tests with regard to the recovery, precision and sensitivity (detection and quantification limit) of (TAC) values in human milk.
Material  and methods. TAC values were determined in twenty five samples of human milk obtained from healthy mothers, residents of Gdańsk, on the 14th day postpartum.
Results.  The average TAC of human milk determined by ABTS assay was 19.61 ±3.311 mg TE (Trolox Equivalents)/100 cm3, the average values obtained by the DPPH assay reached 9.95 ±4.36 mg TE/100 cm3. For each milk sample the TAC determined by the ABTS test was significantly higher than the values pro- duced by the DPPH test. The above findings can be attributed to the presence of substances whose spectra overlap with DPPH• spectra. ABTS test was characterised by a higher sensitivity and repeatability of the determination of TAC in human milk compared to the DPPH test.
Conclusions. Comparing the calculated values for the validation parameters of both methods and taking into account the solubility of DPPH only in polar matrices, slower reaction of selected antioxidants with DPPH radical, and the presence in human milk constituents absorbing electromagnetic radiation in the absorption of DPPH be assumed that the ABTS test is more appropriate method of  determining of TAC in breast milk.

Keywords: human milk, total antioxidant capacity, ABTS test, DPPH test
pub/.pdf Full text available in english in Adobe Acrobat format:
http://www.food.actapol.net/issue1/volume/9_1_2012.pdf

For citation:

MLA Martysiak-Żurowska, Dorota, and Weronika Wenta. "A comparison of abts and dpph methods for assessing the total antioxidant capacity of human milk." Acta Sci.Pol. Technol. Aliment. 11.1 (2012): 83-89.
APA Martysiak-Żurowska D. (2012). A comparison of abts and dpph methods for assessing the total antioxidant capacity of human milk. Acta Sci.Pol. Technol. Aliment. 11 (1), 83-89
ISO 690 MARTYSIAK-ŻUROWSKA, Dorota, WENTA, Weronika. A comparison of abts and dpph methods for assessing the total antioxidant capacity of human milk. Acta Sci.Pol. Technol. Aliment., 2012, 11.1: 83-89.