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Issue 11 (3) 2012 pp. 249-258

Abdalbasit Adam Mariod1, Siddig Ibrahim Abdelwahab2, Sara Elkheir1, Yousif Mohamed Ahmed1, Putri Narrima Mohd Fauzi3, Cheah Shiau Chuen3

1Food Science and Technology Department, College of Agricultural Studies, Sudan University of Science and Technology Khartoum North, Sudan
2
UPM-MAKNA Cancer Research Laboratory, Institute of Bioscience, University Putra Malaysia Serdang,Selangor, Malaysia
3
Centre of Natural Products and Drug Discovery (CENAR), Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia

Antioxidant activity of different parts from Annona squamosa, and Catunaregam nilotica methanolic extract

Abstract

 Background. We extracted phenolic compounds from Annona squamosa (leaves, bark, roots and seedcake), and Catunaregam nilotica (leaves, bark and seedcake) using methanol and their antioxidant activity was evaluated employing various established in vitro systems.
Material and methods. Annona squamosa (leaves, bark, roots and seedcake), and Catunaregam nilotica (leaves, bark and seedcake) were used in the study. Antioxidant activity was estimated using oxygen radical absorbance capacity, MTTassay and DPPH assays, and polyphenols profile was determined by HPLC method.
Results. The total phenolic content was determined by Folin-Ciocalteu method and the highest amounts were 171.5, 170.4, 169.5, and 167.9 g/kg plant extract as GAE for A. squamosa roots, C. nilotica bark, C. nilotica leaves, and A. squamosa bark, respectively. The leaves extracts of the two trees showed high flavoniod content. The results showed that C. nilotica and A. squamosa extracts displayed antioxidant activities, with IC50 values ranging from 7.81 to 62.5 and from 7.81 to 125.0 μg/ml, respectively using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The different parts extracts from two trees showed good antioxidant activity evaluated by oxygen radical absorbance capacity and MTTassay systems.
Conclusion. These results suggested that Annona squamosa and Catunaregam nilotica phenolic compounds could be utilized as a natural antioxidant.

Keywords: Annona squamosa, antioxidant activity, Catunaregam nilotica, 1,1-diphenyl-2-picrylhydrazyl (DPPH), oxygen radical absorbance capacity (ORAC), cytotoxic activity, MTT Assay
pub/.pdf Full text available in english in Adobe Acrobat format:
http://www.food.actapol.net/issue3/volume/4_3_2012.pdf

For citation:

MLA Mariod, Abdalbasit Adam, et al. "Antioxidant activity of different parts from Annona squamosa, and Catunaregam nilotica methanolic extract." Acta Sci.Pol. Technol. Aliment. 11.3 (2012): 249-258.
APA Mariod A.A., Abdelwahab S.I., Elkheir S., Ahmed J.M., Fauzi P.N.M., Chuen Ch.S., (2012). Antioxidant activity of different parts from Annona squamosa, and Catunaregam nilotica methanolic extract. Acta Sci.Pol. Technol. Aliment. 11 (3), 249-258
ISO 690 MARIOD, Abdalbasit Adam, et al. Antioxidant activity of different parts from Annona squamosa, and Catunaregam nilotica methanolic extract. Acta Sci.Pol. Technol. Aliment., 2012, 11.3: 249-258.