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Issue 11 (3) 2012 pp. 293-302

Farooq Anwar1, Roman Przybylski2

1Department of Chemistry, University of Sargodha, Pakistan
2
Department of Chemistry and Biochemistry, University of Lethbridge, Canada

Effect of solvents extraction on total phenolics and antioxidant activity of extracts from flaxseed (Linum usitatissimum L.)

Abstract

 Background. Plant origin food ingredients are the main source of very potent antioxidants. Tocopherols, the main oilseeds natural antioxidants are very potent and when implemented into cell membranes are able to scavenge large number of free radicals. Among plant antioxidants are mainly phenolics, large and diversified group of chemical compounds with different radical scavenging potential.

Material and methods. Defatted flaxseed meals were extracted with pure alcohols and its mixture with water. Acquired extracts were analysed for the content of phenolics and flavonoids using colorimetric procedures. Antioxidative capacity was assessed by utilizing: DPPH stable free radicals; inhibition of linoleic acid oxidation and reducing power of components.
Results. Investigation was conducted on two different batches of flaxseed, assessing antioxidant capacity of compounds extracted with different polarity solvents and extracts were tested for antioxidant activity with different methods. The highest yield of extraction was achieved with 80% methanol but the extract did not contain the highest amount of phenolics and flavonoids. When 80% ethanol was used for extraction the highest amount of flavonoids was detected and also the best antioxidant capacity.
Conclusions. The results clearly showed that utilization of polar solvent enable extraction of significant amounts of phenolics and flavonoids. Those components were the most potent antioxidants present in those extracts. Content of these compounds correlated well with results from applied methods for antioxidant assessment.

 

Keywords: flaxseeds, solvent extraction, antioxidant, total phenolics, total flavonoids
pub/.pdf Full text available in english in Adobe Acrobat format:
http://www.food.actapol.net/issue3/volume/9_3_2012.pdf

For citation:

MLA Anwar, Farooq, and Roman Przybylski. "Effect of solvents extraction on total phenolics and antioxidant activity of extracts from flaxseed (Linum usitatissimum L.)." Acta Sci.Pol. Technol. Aliment. 11.3 (2012): 293-302.
APA Anwar F., Przybylski R. (2012). Effect of solvents extraction on total phenolics and antioxidant activity of extracts from flaxseed (Linum usitatissimum L.). Acta Sci.Pol. Technol. Aliment. 11 (3), 293-302
ISO 690 ANWAR, Farooq, PRZYBYLSKI, Roman. Effect of solvents extraction on total phenolics and antioxidant activity of extracts from flaxseed (Linum usitatissimum L.). Acta Sci.Pol. Technol. Aliment., 2012, 11.3: 293-302.