Wydawnictwo Uniwersytetu Przyrodniczego w Poznaniu

Biblioteka Uniwersytetu Przyrodniczego w Poznaniu

Nauka Przyroda Technologie

Thomson Routers Master List

Scopus

Ebsco

Directory of Open Acces Journals

CBR- Open Access Journals in Poland

FOOD SCIENCE CENTRAL

Index Copernicus Journal Master List

Pubmed Medline

Polska Bibliografia Naukowa

Creaive Commons

Creative Commons licence CC BY-NC (Attribution-NonCommercial)
original articleIssue 13 (4) 2014 pp. 375-383

Chakkere Shivamadhu Madhu1, Honnayakanahalli Marichenne Gowda Manukumar2, Puttalingaiah Basavaraju3

1Department of Biochemistry, Yuvaraja&#
8

2
1
7
;s College, University of Mysore, India
2
Pristine Laboratories, Kodigehalli Gate, Karnataka, India
3
Department of Biotechnology, Manasagangothri, University of Mysore, India

New-vista in finding antioxidant and anti-inflammatory property of crude protein extract from Sauropus androgynus leaf

Abstract

Aim. This study describes antioxidant and anti-inflammatory properties of Sauropus androgynus leaf dialysed protein extract. Free radicals are implicated for many diseases including diabetes mellitus, arthritis, cancer, ageing etc. In the treatment of these diseases, antioxidant therapy has gained utmost importance. The main objective of the present study was to reveal the antioxidant and anti-inflammatory potentiality from S. androgynus leaf dialysed protein extract.
Material and methods. The antioxidant and anti-inflammatory properties of S. androgynus studied using different models viz. hydroxyl radical scavenging, DPPH radical scavenging, reducing power assay, superoxide radical scavenging activity by alkaline DMSO and phosphomolybdenum antioxidant assays and in vitro antiinflammatory activity by inhibition of protein denaturation, membrane stabilisation test models carried out.
Results. Antioxidant activity was estimated in dose dependent manner showed hydroxyl radical (55.62%), DPPH (50%), reducing power (0.286 Abs), alkaline DMSO (72.51%) and phosphomolybdenum (0.198 Abs) activity high at 50 μg/ml concentration compared to standard curcumin (62.31%), curcumin (56.61%), tannic acid (0.54 Abs), curcumin (75.38%) and vitamin E (0.15 Abs) respectively. In-vitro anti-inflammatory activity by hypotonic induced model showed maximum protection (74.17%) compared to standard Acetylsalcylic acid (86.88%) at 100 μg/ml concentration and also in protein denaturation model protected protein denaturation maximum (83.60%) compared to standard Diclofenac (86.82%) at 100 μg/ml concentration respectively.
Conclusion. The antioxidant property usually studied related to the polyphenols and flavonoids present in the extract but present finding concluded that S. androgynus giving hint even potential proteins can also show responsible action effective against free radical mediated disease.

Keywords: antioxidant, anti-inflammatory, neutrophil, lysosomal, erythrocyte
pub/.pdf Full text available in english in Adobe Acrobat format:
http://www.food.actapol.net/issue4/volume/4_4_2014.pdf

http://dx.doi.org/10.17306/J.AFS.2014.4.4

For citation:

MLA Madhu, Chakkere Shivamadhu, et al. "New-vista in finding antioxidant and anti-inflammatory property of crude protein extract from Sauropus androgynus leaf." Acta Sci.Pol. Technol. Aliment. 13.4 (2014): 375-383. http://dx.doi.org/10.17306/J.AFS.2014.4.4
APA Madhu C.S., Manukumar H.M., Puttalingaiah B., (2014). New-vista in finding antioxidant and anti-inflammatory property of crude protein extract from Sauropus androgynus leaf. Acta Sci.Pol. Technol. Aliment. 13 (4), 375-383 http://dx.doi.org/10.17306/J.AFS.2014.4.4
ISO 690 MADHU, Chakkere Shivamadhu, MANUKUMAR, Honnayakanahalli Marichenne Gowda, BASAVARAJU, Puttalingaiah. New-vista in finding antioxidant and anti-inflammatory property of crude protein extract from Sauropus androgynus leaf. Acta Sci.Pol. Technol. Aliment., 2014, 13.4: 375-383. http://dx.doi.org/10.17306/J.AFS.2014.4.4