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Quoc P.T., Van Muoi N., 2016. MICROWAVE-ASSISTED EXTRACTION OF PHENOLIC COMPOUNDS FROM POLYGONUM MULTIFLORUM THUNB. ROOTS. Acta Sci.Pol. Technol. Aliment. 15 (2), 181-189

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original articleIssue 15 (2) 2016 pp. 181-189

Le Pham Tan Quoc1,2, Nguyen Van Muoi1

1Department of Food Technology, Can Tho University, Vietnam
2
Institute of Biotechnology and Food Technology, Industrial University of Ho Chi Minh City, Vietnam

MICROWAVE-ASSISTED EXTRACTION OF PHENOLIC COMPOUNDS FROM POLYGONUM MULTIFLORUM THUNB. ROOTS

Abstract

Background. The aim of this study was to determine the best extraction conditions for total phenolic content (TPC) and antioxidant capacity (AC) of Polygonum multiflorum Thunb. root using microwave-assisted extraction (MAE).
Material and methods. The raw material used was Polygonum multiflorum Thunb. root powder. Five factors such as solvent type, solvent concentrations, solvent/material ratio, extraction time and microwave power were studied; TPC and AC values were determined by the Folin-Ciocalteu method and DPPH free radical scavenging activity measurement, respectively. In addition, studies involved assaying the HPLC test of extracts and SEM of samples.
Results. Optimal results pointed to acetone as the solvent, acetone concentration of 60%, solvent/material ratio of 40/1 (v/w), extraction time of 5 mins and microwave power of 127 W. TPC and AC obtained were approximates 44.3 ±0.13 mg GAE/g DW and 341.26 ±1.54 μmol TE/g DW, respectively. The effect of microwaving on the cell destruction of Polygonum multiflorum Thunb. root was observed by scanning electron microscopy (SEM). Some phenolic compounds were determined by the HPLC method, for instance, gallic acid, catechin and resveratrol.
Conclusion. These factors significantly affected TPC and AC. We can use acetone as a solvent with microwave-assisted extraction to achieve the best result.

Keywords: antioxidant capacity, microwave-assisted extraction, Polygonum multiflorum Thunb., solvent, total polyphenol, Trolox
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http://dx.doi.org/10.17306/J.AFS.2016.2.18

For citation:

MLA Quoc, Le Pham Tan, and Nguyen Van Muoi. "MICROWAVE-ASSISTED EXTRACTION OF PHENOLIC COMPOUNDS FROM POLYGONUM MULTIFLORUM THUNB. ROOTS." Acta Sci.Pol. Technol. Aliment. 15.2 (2016): 181-189. http://dx.doi.org/10.17306/J.AFS.2016.2.18
APA Quoc P.T., Van Muoi N. (2016). MICROWAVE-ASSISTED EXTRACTION OF PHENOLIC COMPOUNDS FROM POLYGONUM MULTIFLORUM THUNB. ROOTS. Acta Sci.Pol. Technol. Aliment. 15 (2), 181-189 http://dx.doi.org/10.17306/J.AFS.2016.2.18
ISO 690 QUOC, Le Pham Tan, MUOI, Nguyen Van. MICROWAVE-ASSISTED EXTRACTION OF PHENOLIC COMPOUNDS FROM POLYGONUM MULTIFLORUM THUNB. ROOTS. Acta Sci.Pol. Technol. Aliment., 2016, 15.2: 181-189. http://dx.doi.org/10.17306/J.AFS.2016.2.18