original articleIssue 16 (1) 2017 pp. 53-67
Marzanna Hęś1, Anna Gliszczyńska-Świgło2, Anna Gramza-Michałowska1
2Department of Technology and Instrumental Analysis, Poznań University of Economics, Poland
The effect of antioxidants on quantitative changes of lysine and methionine in linoleic acid emulsions at different pH conditions
Background. Plants are an important source of phenolic compounds. The antioxidant capacities of green tea, thyme and rosemary extracts that contain these compounds have been reported earlier. However, there is a lack of accessible information about their activity against lipid oxidation in emulsions and inhibit the interaction of lipid oxidation products with amino acids. Therefore, the influence of green tea, thyme and rosemary extracts and BHT (butylated hydroxytoluene) on quantitative changes in lysine and methionine in linoleic acid emulsions at a pH of isoelectric point and a pH lower than the isoelectric point of amino acids was investigated.
Material and methods. Total phenolic contents in plant extracts were determined spectrophotometrically by using Folin-Ciocalteu’s reagent, and individual phenols by using HPLC. The level of oxidation of emulsion was determined using the measurement of peroxides and TBARS (thiobarbituric acid reactive substances). Methionine and lysine in the system were reacted with sodium nitroprusside and trinitrobenzenesulphonic acid respectively, and the absorbance of the complexes was measured.
Results. Extract of green tea had the highest total polyphenol content. The system containing antioxidants and amino acid protected linoleic acid more efficiently than by the addition of antioxidants only. Lysine and methionine losses in samples without the addition of antioxidants were lower in their isoelectric points than below these points. Antioxidants decrease the loss of amino acids. The protective properties of antioxidants towards methionine were higher in a pH of isoelectric point whereas towards lysine in pH below this point. Conclusion. Green tea, thyme and rosemary extracts exhibit antioxidant activity in linoleic acid emulsions. Moreover, they can be utilized to inhibit quantitative changes in amino acids in lipid emulsions. However, the antioxidant efficiency of these extracts seems to depend on pH conditions. Further investigations should be carried out to clarify this issue.
Keywords: lipid oxidation, linoleic acid emulsion, green tea, rosemary and thyme extracts, lysine, methio- nine, environmental pH
|MLA||Hęś, Marzanna, et al. "The effect of antioxidants on quantitative changes of lysine and methionine in linoleic acid emulsions at different pH conditions." Acta Sci.Pol. Technol. Aliment. 16.1 (2017): 53-67. http://dx.doi.org/10.17306/J.AFS.2017.0455|
|APA||Hęś M., Gliszczyńska-Świgło A., Gramza-Michałowska A. (2017). The effect of antioxidants on quantitative changes of lysine and methionine in linoleic acid emulsions at different pH conditions. Acta Sci.Pol. Technol. Aliment. 16 (1), 53-67 http://dx.doi.org/10.17306/J.AFS.2017.0455|
|ISO 690||Hęś, Marzanna, GLISZCZYńSKA-ŚWIGłO, Anna, GRAMZA-MICHAłOWSKA, Anna. The effect of antioxidants on quantitative changes of lysine and methionine in linoleic acid emulsions at different pH conditions. Acta Sci.Pol. Technol. Aliment., 2017, 16.1: 53-67. http://dx.doi.org/10.17306/J.AFS.2017.0455|