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original articleIssue 16 (4) 2017 pp. 403-412

He Chen1, Jie Huang1, Xiaoyu Shi1, Yichao Li2, Yu Liu2

1School of Food and Biological Engineering, Shaanxi University of Science and Technology Xi?an,China
2
Shaanxi Heshi Dairy, China

Effects of six substances on the growth and freeze-drying of Lactobacillus delbrueckii subsp. bulgaricus

Abstract

Background. The efficacy of Lactobacillus delbrueckii subsp. bulgaricus as starter cultures for the dairy industry depends largely on the number of viable and active cells. Freeze-drying is the most convenient and successful method to preserve the bacterial cells. However, not all strains survived during freeze-drying.

Methods. The effects of six substances including NaCl, sorbitol, mannitol, mannose, sodium glutamate, betaine added to the MRS medium on the growth and freeze-drying survival rate and viable counts of Lb. delbrueckii subsp. bulgaricus were studied through a single-factor test and Plackett-Burman design. Subsequently, the optimum freeze-drying conditions of Lb. delbrueckii subsp. bulgaricus were determined.

Results. Lb. delbrueckii subsp. bulgaricus survival rates were up to the maximum of 42.7%, 45.4%, 23.6%, while the concentrations of NaCl, sorbitol, sodium glutamate were 0.6%, 0.15%, 0.09%, respectively. In the optimum concentration, the viable counts in broth is 6.1, 6.9, 5.13 (×108 CFU/mL), respectively; the viable counts in freeze-drying power are 3.09, 5.2, 2.7 (×1010 CFU/g), respectively.

Conclusion. Three antifreeze factors including NaCl, sorbitol, sodium glutamate have a positive effect on the growth and freeze-drying of Lb. delbrueckii subsp. bulgaricus. The results are beneficial for developing Lb. delbrueckii subsp. bulgaricus.

Keywords: Lactobacillus delbrueckii subsp. bulgaricus, antifreeze factors, freeze-drying, single-factor test, PB screening test
pub/.pdf Full text available in english in Adobe Acrobat format:
http://www.food.actapol.net/issue4/volume/5_4_2017.pdf

http://dx.doi.org/10.17306/J.AFS.0512

For citation:

MLA Chen, He, et al. "Effects of six substances on the growth and freeze-drying of Lactobacillus delbrueckii subsp. bulgaricus." Acta Sci.Pol. Technol. Aliment. 16.4 (2017): 403-412. http://dx.doi.org/10.17306/J.AFS.0512
APA Chen H., Huang J., Shi X., Li Y., Liu Y. (2017). Effects of six substances on the growth and freeze-drying of Lactobacillus delbrueckii subsp. bulgaricus. Acta Sci.Pol. Technol. Aliment. 16 (4), 403-412 http://dx.doi.org/10.17306/J.AFS.0512
ISO 690 CHEN, He, et al. Effects of six substances on the growth and freeze-drying of Lactobacillus delbrueckii subsp. bulgaricus. Acta Sci.Pol. Technol. Aliment., 2017, 16.4: 403-412. http://dx.doi.org/10.17306/J.AFS.0512