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Górnaś P., Sieger A., Dwiecki K., Nogala-Kałucka M., Palewski K., 2006. Determination of tocopherols content in sunflower oil during oxidation using fluorescence technique. Acta Sci.Pol. Technol. Aliment. 5 (2), 157-164

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Issue 5 (2) 2006 pp. 157-164

Paweł Górnaś, Aleksander Sieger, Krzysztof Dwiecki, Małgorzata Nogala-Kałucka, Krzysztof Palewski

Determination of tocopherols content in sunflower oil during oxidation using fluorescence technique

Abstract The fluorescence intensity of tocopherols originated from cold-pressed sunflower oil at different degree of oxidation in presence of 1,2-dipalmitylo-sn-glicero-3- -fosfatidylocholine lipid (DPPC) membrane have been measured. It has been shown that fluorescence of tocopherol depends on amount of oil as well as on membrane concentra-tion in the sample. Addition of oil with increasing peroxide value PV decreased the toco-pherol fluorescence due to its disappearance in the sample. At constant membrane con-centration chromatographically determined the amount of tocopherol in oil sample corre-lated with peroxide value and fluorescence intensity. Such results allowed us construct calibration curve which may be used for fast and accurate peroxide value determination using fluorescence technique.
Keywords: sunflower oil, tocopherols, peroxide value, DPPC membrane, fluorescence
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For citation:

MLA Górnaś, Paweł, et al. "Determination of tocopherols content in sunflower oil during oxidation using fluorescence technique." Acta Sci.Pol. Technol. Aliment. 5.2 (2006): 157-164.
APA Górnaś P., Sieger A., Dwiecki K., Nogala-Kałucka M., Palewski K. (2006). Determination of tocopherols content in sunflower oil during oxidation using fluorescence technique. Acta Sci.Pol. Technol. Aliment. 5 (2), 157-164
ISO 690 GóRNAś, Paweł, et al. Determination of tocopherols content in sunflower oil during oxidation using fluorescence technique. Acta Sci.Pol. Technol. Aliment., 2006, 5.2: 157-164.