ACTA Scientiarum Polonorum - Technologia Alimentaria

abstract: The fluorescence intensity of tocopherols originated from cold-pressed sunflower oil at different degree of oxidation in presence of 1,2-dipalmitylo-sn-glicero-3- -fosfatidylocholine lipid (DPPC) membrane have been measured. It has been shown that fluorescence of tocopherol depends on amount of oil as well as on membrane concentra-tion in the sample. Addition of oil with increasing peroxide value PV decreased the toco-pherol fluorescence due to its disappearance in the sample. At constant membrane con-centration chromatographically determined the amount of tocopherol in oil sample corre-lated with peroxide value and fluorescence intensity. Such results allowed us construct calibration curve which may be used for fast and accurate peroxide value determination using fluorescence technique.