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Issue 6 (1) 2007 pp. 5-16

Magdalena Montowska, Edward Pospiech

Agricultural University of Poznan

SPECIES IDENTIFICATION OF MEAT BY ELECTROPHORETIC METHODS

Abstract Electrophoretic methods can be used to identify meat of various animal species. The protein electrophoresis, especially the IEF of the sarcoplasmic proteins, is a well-established technique for species identification of raw fish and is used in the control of seafood authenticity. However, in the case of the analysis of heat-processed fish, the method is applicable only to those species which possess characteristic patterns of the heat-stable parvalbumins. Heat-denatured fish muscle proteins may be solubilised by urea or sodium dodecylsulfate (SDS) and separated by urea-IEF or SDS-PAGE, respectively. The comparison of these two methods allowed to conclude that, basically, each of them can be used for species identification of heated fishery products. However, extensively washed products may be preferentially analysed by the SDS-PAGE, because most of the parvalbumins are washed out leaving mainly myosins. On the other hand, the IEF method may be preferred for the differentiation of closely related species rich in parvalbumins isoforms. It is evident from the literature data that species-specific protein separations yield proteins of low molecular weight made up of three light chains of myosin (14-23 kDa), troponin (19-30 kDa) and parvalbumin (about 12 kDa). Investigations showed that the SDS-PAGE method can be used to identify meats of: cattle, sheep, lambs, goats, red deer and rabbits. The technique allowed researchers to identify the following myofibrillar and sarcoplasmic muscle proteins: myosin and actin, α-actinin, tropomyosin, troponin. SDS-PAGE allowed the identification of myofibrillar proteins taking into account their molecular weights which was not possible with the assistance of the PAGIF because too many protein bands were obtained. It was possible to obtain differences in the separation of proteins characteristic for certain species, e.g. beef, resulting from the presence of sin-gle myofibrillar proteins.
Keywords: IEF, SDS-PAGE, species identification
pub/.pdf Full text available in english in Adobe Acrobat format:
http://www.food.actapol.net/issue1/volume/1_1_2007.pdf

For citation:

MLA Montowska, Magdalena, and Edward Pospiech. "SPECIES IDENTIFICATION OF MEAT BY ELECTROPHORETIC METHODS." Acta Sci.Pol. Technol. Aliment. 6.1 (2007): 5-16.
APA Montowska M., Pospiech E. (2007). SPECIES IDENTIFICATION OF MEAT BY ELECTROPHORETIC METHODS. Acta Sci.Pol. Technol. Aliment. 6 (1), 5-16
ISO 690 MONTOWSKA, Magdalena, POSPIECH, Edward. SPECIES IDENTIFICATION OF MEAT BY ELECTROPHORETIC METHODS. Acta Sci.Pol. Technol. Aliment., 2007, 6.1: 5-16.