ACTA Scientiarum Polonorum

Dietary polyphenols are considered beneficial because of their potential protective role in the pathogenesis of chronic diseases associated to oxidative stress. However, many of these effects may depend on the concentration of the antioxidants used. The aim of the study was to evaluate the effect of quercetin, chlorogenic acid and epigallocatechin on proliferation of human colon adenocarcinoma cells Caco-2. Caco-2 cells were treated with different concentrations (25, 50, 100, 200, and 400 μM in DMSO) of antioxidants and the proliferation was assayed by MTT method. The results of the study indicated that chlorogenic acid and quercetin enhanced Caco-2 cells proliferation in time- and concentration-dependent manner. In contrary, epigallocatechin exhibit inhibitory influence, particularly significant at concentration ≥ 200 μM.