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Issue 8 (3) 2009 pp. 47-58

Gabriela Paun Roman1, Elena Neagu1, Gabriel Lucian Radu2

National Institute of Research and Development for Biological Sciences in Bucharest Polytechnic University of Bucharest

Antiradical activities of Salvia officinalis and Viscum album L. extracts concentrated by ultrafiltration process

Abstract

 

Background. In the present study the antioxidant properties were investigated of the medicinal plants Salvia officinalis L. (Labiaceae family) and Viscum album L. (Loranthaceae), both of them known for a long time as a remedy in the traditional medicine. The aim
of this study was to prove the efficiency of ultrafiltration process for the concentration
of herbs extracts and to evaluate the concentrate’s antioxidant activity.
Material and methods. The extracts were prepared by maceration, using different solvents. After filtering the extract through Isolab quantitative filter paper “medium”, each of the filtrates was processed by microfiltration (MF; Millipore filters with 45 μm), followed by ultrafiltration (UF). The regenerated cellulose (Millipore), polysulfone and polyacrylonitrile ultrafiltration membranes were used in the experiment. The initial extracts and samples of permeate and retentate after ultrafiltration of extracts have been characterized by determination of the protein total and total phenolic content. Standard methods like ABTS and DPPH assay are used to measure the antioxidant activity.
Results. For the three types of tested membranes: Millipore, PSF and PAN, PAN membrane proves to have the greatest efficiency since it shows the highest permeate flux and the greatest retention degree for bioactive compounds. The concentrated extracts obtained after ultrafiltration with polyacrylonitrile membrane had the strongest scavenging activity for all extracts.
Conclusions. The results of this study has revealed that the concentrated extracts have
a very high radical scavenging activity (TEAC values for sage hydro-alcoholic concentrated extracts in range 351.87-479.04 μmol Trolox/mL extract and for mistletoe concentrated extract E2 in range 345.14-426.18 μmol Trolox/mL extract; the DPPH inhibition values was over 85% for S. officinalis concentrated extracts and ranges between 66.2% and 88.2% DPPH inhibition for V. album concentrated), therefore can be considered as a good source for further medicinal applications.

 

Keywords: Salvia officinalis, Viscum album, ultrafiltration, antioxidant activity, ABTS, DPPH
pub/.pdf Full text available in english in Adobe Acrobat format:
http://www.food.actapol.net/issue3/volume/5_3_2009.pdf

For citation:

MLA Roman, Gabriela Paun, et al. "Antiradical activities of Salvia officinalis and Viscum album L. extracts concentrated by ultrafiltration process." Acta Sci.Pol. Technol. Aliment. 8.3 (2009): 47-58.
APA Roman G.P., Neagu E., Radu G.L. (2009). Antiradical activities of Salvia officinalis and Viscum album L. extracts concentrated by ultrafiltration process. Acta Sci.Pol. Technol. Aliment. 8 (3), 47-58
ISO 690 ROMAN, Gabriela Paun, NEAGU, Elena, RADU, Gabriel Lucian. Antiradical activities of Salvia officinalis and Viscum album L. extracts concentrated by ultrafiltration process. Acta Sci.Pol. Technol. Aliment., 2009, 8.3: 47-58.