Antioxidant potential of herbs extracts and impact on HepG2 cells viability
Mercury poisoning is responsible for inducing serious adverse effects in living organisms. One of protection factors could be substances proven to possess high antioxidant and metal chelating activity – plant polyphenols. There are many sources of polyphenols in plant kingdom but the most interesting for food industry could be widely consumed herbs. Aim of the research was to evaluate antioxidative potential of selected plant extracts and its influence on HepG2 cells in different conditions. Ethanolic herbs extracts were characterised by total polyphenol content. Antioxidant activity was estimated with use of DPPH• and ABTS+• radicals scavenging methods and FRAP. Research included cells viability estimation by the MTT assay and cells exposition to HgCl2, chemical agent inducing cell death. Analysis of herbs extracts antioxidative activity showed best potential represented thyme and marjoram, highest FRAP was evaluated in samples with mint and marjoram extracts. On the basis of received results it was found that examined plant extracts showed weak protection against Hg presence in examined cells environment.
herbs, plant extracts, cytotoxicity, HepG2 cells, FRAP, radicals
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Poznań University of Life Sciences, Poland
Anna
Gramza-Michałowska
University of British Columbia Vancouver, Canada
Zyta
Abramowski
University of British Columbia Vancouver, Canada
Eduardo
Jovel
Poznań University of Life Sciences, Poland
Marzanna
Hes