The flow cytometric analysis of lupin proteins’ potential to induce the respiratory burst in human neutrophils
There is an ever-increasing demand for lupin seed protein in human nutrition. However, their usage as a food ingredient can be limited by their influence on the immune system. The aim of the study was to analyse, by means of flow cytometry, the potential of lupin seed globulins to induce the respiratory burst in human neutrophils. The results proved the potential of lupin globulins to induce the increased ROS production in human neutrophils. The increased ROS production by the cells treated with lupin globulins and, at the same time, stimulated with the PMA may suggest a synergistic effect of the globulins and the PMA.
lupin globulins, immunoreactivity, respiratory burst, reactive oxygen species, neutrophils, phorbol 12-myristate 13-acetate
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Poznań University of Life Sciences
Patrycja
Kłos
University of Medical Sciences in Poznań
Barbara
Poniedziałek
Poznań University of Life Sciences
Eleonora
Lampart-Szczapa
Poznań University of Life Sciences
Justyna
Gralik
University of Medical Sciences in Poznań
Krzysztof
Wiktorowicz