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Issue 11 (1) 2012 pp. 19-25

Agnieszka Drożdżyńska1, Krzysztof Dziedzic2, Alicja Kośmider1, Katarzyna Leja1, Katarzyna Czaczyk1, Danuta Górecka2

1Department of Biotechnology and Food Microbiology, Poznań University of Life Sciences, Poland
2
Department of Food Service and Catering, Poznań University of Life Sciences, Poland

Application of fast liquid chromatography for antioxidants analysis

Abstract

Background. An intensive development of the Fast Liquid Chromatography (FLC) has been recently observed. It makes possible to reduce time analysis and improve resolution as well as sensitivity. The aim of this study was to separate the chosen antioxidants optimization using the FLC method.
Material  and methods. The three various procedures for antioxidants analysis were compared. Mobile phases containing aqueous solution of formic acid, acetic acid, acetonitrile, and methanol were tested. Limit of detection (LOD), limit of quantification (LOQ), linearity and repeatability of each procedures were determined.
Results. Developed procedure enabled to separate all analytes and allowed to get low LOD levels and good repeatability. This procedure was used for antioxidants analysis in buckwheat and buckwheat products.
Conclusion. Fast Liquid Chromatography allows to  reduce time analysis and  obtain good  validation parameters.

Keywords: Fast Liquid Chromatography, antioxidants
pub/.pdf Full text available in english in Adobe Acrobat format:
http://www.food.actapol.net/issue1/volume/2_1_2012.pdf

For citation:

MLA Drożdżyńska, Agnieszka, et al. "Application of fast liquid chromatography for antioxidants analysis." Acta Sci.Pol. Technol. Aliment. 11.1 (2012): 19-25.
APA Drożdżyńska A., Dziedzic K., Kośmider A., Leja K., Czaczyk K., Górecka D. (2012). Application of fast liquid chromatography for antioxidants analysis. Acta Sci.Pol. Technol. Aliment. 11 (1), 19-25
ISO 690 DROżDżYńSKA, Agnieszka, et al. Application of fast liquid chromatography for antioxidants analysis. Acta Sci.Pol. Technol. Aliment., 2012, 11.1: 19-25.