• Thomson Routers Master List
  • Scopus
  • Pubmed Medline
  • Ebsco
  • CAB Abstracts
  • FOOD SCIENCE CENTRAL
  • Directory of Open Acces Journals
  • Google Scholar
  • Index Copernicus Journal Master List
  • Polska Bibliografia Naukowa
  • AGRO
  • Arianta
  • CBR- Open Access Journals in Poland
  • Wydawnictwo Uniwersytetu Przyrodniczego w Poznaniu
  • Biblioteka Uniwersytetu Przyrodniczego w Poznaniu
  • Creaive Commons
Creative Commons licence CC BY-NC (Attribution-NonCommercial)
original articleIssue 17 (1) 2018 pp. 11-18

Marianna Raczyk1, Anna Paszel-Jaworska2, Magdalena Rudzińska1

1Faculty of Food Science and Nutrition, Poznań University of Life Sciences, Poland
2
Department of Clinical Chemistry and Molecular Diagnostics, Poznan University of Medical Sciences, Poland

Cytotoxic activity of stigmasteryl esters and products of their thermo-oxidative degradation against drug sensitive and drug resistant human acute lymphoblastic leukemia cells

Abstract

Background. Phytosterols are mainly known as a cholesterol-lowering factor, although they form oxidation products during food storage and processing. Moreover, phytosterol oxidation products (POP) can be ab- sorbed and found in human serum, so there is the need to investigate their impact on different kinds of cells. Material and methods. Esters of fatty acids (oleic, linoleic and linolenic) with stigmasterol were synthetized and heated at 180°C, for 1–12 hours. The cytotoxic effect on the leukemic cells of unheated stigmasteryl esters and the mixture of compounds after heating was determined using MTT assays. POP were identified using GC-MS. The total number of POP was analysed by SPE fractionation and GC-FID separation. Dimers, trimers and oligomers in non-polar fraction were determined by gel permeation chromatography with refractive index detection.

Results. After heating, stigmasterol oxidation products were formed (up to 1.1 mg/g ester). The heating increased the potency of the compounds to reduce cell population and form POPs and oligomers in a time-dependent manner.

Conclusion. The cytotoxicity depends on the kind of ester, dose and time. The strongest cytotoxic effect was found after 72 hours of cell treatment. Among the three stigmasteryl esters tested the most cytotoxic effect was caused by stigmasteryl linoleate.

Keywords: stigmasterol, fatty acid, ester, oxidation, oligomers, cytotoxicity
pub/.pdf Full text available in english in Adobe Acrobat format:
http://www.food.actapol.net/issue1/volume/2_1_2018.pdf

http://dx.doi.org/10.17306/J.AFS.0516

For citation:

MLA Raczyk, Marianna, et al. "Cytotoxic activity of stigmasteryl esters and products of their thermo-oxidative degradation against drug sensitive and drug resistant human acute lymphoblastic leukemia cells." Acta Sci.Pol. Technol. Aliment. 17.1 (2018): 11-18. http://dx.doi.org/10.17306/J.AFS.0516
APA Raczyk M., Paszel-Jaworska A., Rudzińska M. (2018). Cytotoxic activity of stigmasteryl esters and products of their thermo-oxidative degradation against drug sensitive and drug resistant human acute lymphoblastic leukemia cells. Acta Sci.Pol. Technol. Aliment. 17 (1), 11-18 http://dx.doi.org/10.17306/J.AFS.0516
ISO 690 RACZYK, Marianna, PASZEL-JAWORSKA, Anna, RUDZIńSKA, Magdalena. Cytotoxic activity of stigmasteryl esters and products of their thermo-oxidative degradation against drug sensitive and drug resistant human acute lymphoblastic leukemia cells. Acta Sci.Pol. Technol. Aliment., 2018, 17.1: 11-18. http://dx.doi.org/10.17306/J.AFS.0516