Acta Scientiarum Polonorum Technologia Alimentaria

ISSN:1644-0730, e-ISSN:1898-9594

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original articleIssue 17 (4) 2018 pp. 313-319

Ling Wan, Gang Chen, SuPing Jian, Xiao Jie Yin, HongLin Zhu

State Key Laboratory of Food Science and Technology, Nanchang University, China

Antioxidant and xanthine oxidase inhibitory properties and LC-MS/MS identification of compoundsof ethanolic extract from Mulberry leaves

Abstract

Background. Xanthine oxidase (XO) enzyme inhibitors are used to treat hyperuricemia. Certain natural substances have been reported to be strong inhibitors of xanthine oxidase. The purpose of this study was to evaluate the antioxidant activity and XO inhibition effect of ethanolic extract from mulberry leaves.

Material and methods. The chemical composition of Mulberry leaf ethanolic extract (MLEE) was identi- fied by the LC-MS/MS method. Antioxidant activity was measured by three different assays (i.e. superoxide, hydrogen peroxide, and hydroxyl radicals assays). The inhibitory effect on XO and its inhibitory mechanism were investigated through the testing of inhibition kinetics and inhibition rate.

Results. MLEE showed significant antioxidant activity in the superoxide, hydrogen peroxide, and hydroxyl radicals assays (IC50 of 0.33 ±0.006 mg/mL, 45 ±0.8 μg/mL, and 2.54 ±0.05 mg/mL for each assay respec- tively). The effects of XO inhibitory activity showed that MLEE was a reversible and competitive inhibitor with IC50 values of 1104.76 ±7.1 μg/mL. Eleven compounds, including six flavonoids and five phenolic acids, were identified.

Conclusion. MLEE has been shown to exhibit antioxidant activity and XO inhibitory effect, which provides a new insight into the properties of mulberry leaves and their potential future applications as a natural anti- gout drug.

Keywords: mulberry leaves, antioxidant, xanthine oxidase, LC-MS/MS
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http://www.food.actapol.net/issue4/volume/2_4_2018.pdf

https://doi.org/10.17306/J.AFS.2018.0587

For citation:

MLA Wan, Ling, et al. "Antioxidant and xanthine oxidase inhibitory properties and LC-MS/MS identification of compoundsof ethanolic extract from Mulberry leaves." Acta Sci.Pol. Technol. Aliment. 17.4 (2018): 313-319. https://doi.org/10.17306/J.AFS.2018.0587
APA Wan L., Chen G., Jian S., Yin H. J., Zhu H. (2018). Antioxidant and xanthine oxidase inhibitory properties and LC-MS/MS identification of compoundsof ethanolic extract from Mulberry leaves. Acta Sci.Pol. Technol. Aliment. 17 (4), 313-319 https://doi.org/10.17306/J.AFS.2018.0587
ISO 690 WAN, Ling, et al. Antioxidant and xanthine oxidase inhibitory properties and LC-MS/MS identification of compoundsof ethanolic extract from Mulberry leaves. Acta Sci.Pol. Technol. Aliment., 2018, 17.4: 313-319. https://doi.org/10.17306/J.AFS.2018.0587