Acta Scientiarum Polonorum Technologia Alimentaria

ISSN:1644-0730, e-ISSN:1898-9594

Acta Scientiarum Polonorum Logo
Submit manuscript
Journal metrics
Indexed in:
Creative Commons licence CC BY-NC (Attribution-NonCommercial)
original articleIssue 17 (4) 2018 pp. 377-385

Spandan A. Chaudhary1, Pooja S. Chaudhary1, Bakhtiyar A. Syed2, Richa Misra1, Prashanth G. Bagali1, Sara Vitalini3, Marcello Iriti3

1Diagnostics and Genomics Research Division, Xcelris Labs Limited, Ahmedabad, Gujarat, India
2
Department of Biotechnology, Hemchandracharya North Gujarat University, India
3
Department of Agricultural and Environmental Sciences, Milan State University, Italy

Validation of a method for diosgenin extraction from fenugreek (Trigonella foenum-graecum L.)

Abstract

Background. Diosgenin is a very important plant secondary metabolite and raw material for the drug indus- try. Plant sources rich in diosgenin include yam (Dioscorea spp.) and fenugreek (Trigonella foenum-graecum L.). A method for diosgenin extraction from yam extracts has previously been validated, but its extraction from fenugreek plants still requires validation. In addition, all available methods require time-consuming additional purification steps. The present study was aimed at developing a low cost, less time-consuming single-step method for diosgenin extraction from fenugreek.

Material and methods. This study represents a method developed for diosgenin extraction from fenugreek plants without any additional/supportive purification methods such as chromatography or thin-layer chroma- tography. Diosgenin yield estimation and purity analysis by HPLC method, along with accuracy and preci- sion analysis, is presented.

Results. Five different fenugreek varieties were subjected to a newly developed diosgenin extraction method, and an HPLC chromatogram showed a single peak corresponding to diosgenin. Yield was determined by the standard curve method. Limit of detection (LOD) and limit of quantification (LOQ) for the assay were found to be 0.0312 and 0.102 μg, respectively; tcalculated for slope and other statistical parameters were found to be significant (P value < 0.001) for this method.

Conclusion. We have developed a fast, accurate and low cost method for diosgenin extraction from fenu- greek. Although the authors have studied this method only in fenugreek plants, it could be applied to the extraction of a few other plant secondary metabolites, which will help researchers to save time and effort.

Keywords: diosgenin, fenugreek, HPLC, LOD, quantification
pub/.pdf Full text available in english in Adobe Acrobat format:
http://www.food.actapol.net/issue4/volume/8_4_2018.pdf

https://doi.org/10.17306/J.AFS.2018.0606

For citation:

MLA Chaudhary, Spandan A., et al. "Validation of a method for diosgenin extraction from fenugreek (Trigonella foenum-graecum L.)." Acta Sci.Pol. Technol. Aliment. 17.4 (2018): 377-385. https://doi.org/10.17306/J.AFS.2018.0606
APA Chaudhary S. A., Chaudhary P. S., Syed B. A., Misra R., Bagali P. G., Vitalini S., Iriti M. (2018). Validation of a method for diosgenin extraction from fenugreek (Trigonella foenum-graecum L.). Acta Sci.Pol. Technol. Aliment. 17 (4), 377-385 https://doi.org/10.17306/J.AFS.2018.0606
ISO 690 CHAUDHARY, Spandan A., et al. Validation of a method for diosgenin extraction from fenugreek (Trigonella foenum-graecum L.). Acta Sci.Pol. Technol. Aliment., 2018, 17.4: 377-385. https://doi.org/10.17306/J.AFS.2018.0606