Acta Scientiarum Polonorum Technologia Alimentaria

ISSN:1644-0730, e-ISSN:1898-9594

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original articleIssue 18 (3) 2019 pp. 269-278

Fangfang Cheng1, He Chen1, Ni Lei1, Meng Zhang2, Hongchang Wan2, Guowei Shu1

1School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an, China
2
Shaanxi Yatai Dairy, China

Effect of prebiotics, inorganic salts and amino acids for cell envelope proteinase production from Lactobacillus plantarum LP69

Abstract

Background. Cell-envelope proteinases (CEPs) can improve the texture and organoleptic characteristics of dairy products, and may also cause the release of bioactive peptides, which contribute to improving the host’s health. Thus, the CEPs with high activity produced by L. plantarum has great potential in the development of functional dairy products.

Methods. A single factor experiment was used to investigate the effects of prebiotics (inulin, stachyose, isomaltooligosaccharide, xylooligosaccharides, galactooligosaccharides and fructooligosaccharides), inorganic salts (Na2HPO4, NaH2PO4, K2HPO4, KH2PO4, CH3COONa and C6H14N2O7) and amino acids (arginine, leucine, serine, lysine, alanine and glutamic acid) on the activity of cell envelope proteinases (CEPs), specific activity, protein content, OD600 value and pH in MRS broth fermented by Lactobacillus plantarum LP69.

Results. The optimum concentration of inulin (0.7%), isomaltooligosaccharide (0.5%), Na2HPO4 (0.4%), CH3COONa (0.4%), leucine (20 mg/L), serine (20 mg/L) for L. plantarum LP69 was estimated with the activity of CEP in the range 17.36–21.47 U/mL, protein content in the range 19.18–22.53 mg/mL, specific activity in the range 0.77–1.12 U/mg.

Conclusion. Inulin, isomaltooligosaccharide, Na2HPO4, CH3COONa, leucine and serine are superior to other selected substances, and have a significant influence on the CEP activity and specific activity of L. plantarum LP69. This would provide a reference for further optimization of CEP-producing media of L. plantarum LP69.

Keywords: Lactobacillus plantarum LP69, cell-envelope proteinases, prebiotics, inorganic salts, amino acids, single-factor test
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https://doi.org/10.17306/J.AFS.2019.0656

For citation:

MLA Cheng, Fangfang, et al. "Effect of prebiotics, inorganic salts and amino acids for cell envelope proteinase production from Lactobacillus plantarum LP69." Acta Sci.Pol. Technol. Aliment. 18.3 (2019): 269-278. https://doi.org/10.17306/J.AFS.2019.0656
APA Cheng F., Chen H., Lei N., Zhang M., Wan H., Shu G. (2019). Effect of prebiotics, inorganic salts and amino acids for cell envelope proteinase production from Lactobacillus plantarum LP69. Acta Sci.Pol. Technol. Aliment. 18 (3), 269-278 https://doi.org/10.17306/J.AFS.2019.0656
ISO 690 CHENG, Fangfang, et al. Effect of prebiotics, inorganic salts and amino acids for cell envelope proteinase production from Lactobacillus plantarum LP69. Acta Sci.Pol. Technol. Aliment., 2019, 18.3: 269-278. https://doi.org/10.17306/J.AFS.2019.0656