Acta Scientiarum Polonorum Technologia Alimentaria

ISSN:1644-0730, e-ISSN:1898-9594

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original articleIssue 18 (3) 2019 pp. 317-332

Fathy H. Elsaid1, Ali A. Khalil1, Eman M. Ibrahim2, Abdelmegeed Mansour1, Abdelaziz M. Hussein3

1Department of Medical Physiology, Faculty of Medicine, Al-Azhar University, Assiut, Egypt
2
Department of Pathology, Faculty of Medicine, Mansoura University, Egypt
3
Department of Medical Physiology, Faculty of Medicine, Mansoura University, Egypt

Effects of exercise and stevia on renal ischemia/reperfusion injury in rats

Abstract

Background. The present work was designed to study the effects of methanolic stevia extracts and aerobic exercise and combination of both on renal I/R injury in male rats.

Methods. 60 adult male Sprague-Dawley rats were subdivided into five equal groups as sham, control, exercise, stevia, and stevia plus exercise group. After 5 weeks of exercise and stevia, animals were exposed to 45 min of left renal ischemia and right nephrectomy followed by reperfusion. Serum creatinine, creatinine clearance, fractional Na excretion (FENa+), malondialdehyde (MDA), reduced glutathione (GSH) and catalase (CAT) levels in kidney tissues were measured. Also, renal histopathology and the expression of caspase-3 by immunohistochemical examination were done.

Results. The results showed that stevia, exercise or combination of stevia and exercise caused a significant decrease in serum level of creatinine (p < 0.001) and FENa+ (p < 0.001) and an increase in creatinine clearance (p < 0.001). Moreover, this caused a significant decrease in (MDA; p < 0.046) and an increase in GSH (p < 0.01) and CAT (p < 0.01), as well as causing a significant decrease in caspase 3 expression compared to the control group.

Conclusion. Pretreatment with either stevia or exercise of combination of both seem to have protective effects on renal I/R injury. However, the protective effect of exercise against renal I/R injury seems to be less than stevia. These effects might be due to attenuation of oxidative stress and apoptosis in kidney tissues.

Keywords: renal ischemia/reperfusion, oxidative stress, Stevia rebaudiana, exercise, caspase 3
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https://doi.org/10.17306/J.AFS.2019.0652

For citation:

MLA Elsaid, Fathy H., et al. "Effects of exercise and stevia on renal ischemia/reperfusion injury in rats." Acta Sci.Pol. Technol. Aliment. 18.3 (2019): 317-332. https://doi.org/10.17306/J.AFS.2019.0652
APA Elsaid F. H., Khalil A. A., Ibrahim E. M., Mansour A., Hussein A. M. (2019). Effects of exercise and stevia on renal ischemia/reperfusion injury in rats. Acta Sci.Pol. Technol. Aliment. 18 (3), 317-332 https://doi.org/10.17306/J.AFS.2019.0652
ISO 690 ELSAID, Fathy H., et al. Effects of exercise and stevia on renal ischemia/reperfusion injury in rats. Acta Sci.Pol. Technol. Aliment., 2019, 18.3: 317-332. https://doi.org/10.17306/J.AFS.2019.0652