Acta Scientiarum Polonorum Technologia Alimentaria

Abstract Cultivation has been performed of isolated earlier low-protease mutants and parental strain in the presence of novel inducers of cellulases production – lactulose and lactobionic acid mixed with lactose in the ratio 1:1. Nine among ten tested during batch cultivation low-protease mutants exhibited lower ability for the production of proteases than parental strain M-7. Higher enzymatic activities have been estimated (cellulolytic, xylanolytic and beta – galaktosidase) of culture filtrates during cultivation in the presence of mixtures of lactobionic acid and lactose in comparison to lactulose and lactose. Tested, during continuous cultivation, low-protease mutant T. reesei Mp5 exhibited high resistance for the temperature shifting. FPU activities of culture filtrates were stable in the high range of temperature of cultivation (26-34°C). In addition current knowledge about correlation between protease and other enzymes of culture fluids of selected filamentous fungi has been reviewed. The ways of preventing homologous and heterologous protein produced by fungi and bacteria from enzymatic cleavage by proteases have been also described. This work is an introduction for future studies on correlation between proteases and other enzymes in culture filtrates of Trichoderma reesei.