Acta Scientiarum Polonorum Technologia Alimentaria

ISSN:1644-0730, e-ISSN:1898-9594

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original articleIssue 21 (2) 2022 pp. 129-138

Tarek Hefzalrahman, Mohamed K. S. Morsi, Nashwa F. S. Morsy, Karima S. M. Hammad

Food Science Department, Faculty of Agriculture, Cairo University, Egypt

Application of enzyme and ultrasound assisted extraction of polyphenols from avocado (Persea americana Mill.) peel as natural antioxidants

Abstract

Background. Hass avocado (Persea americana Mill.) peel is a rich source of natural antioxidants. The present work aims to evaluate ultrasound and enzyme-assisted extraction methods of polyphenols with antioxidant properties from avocado peel.
Materials and methods. The impact of extraction parameters on the extraction yield of polyphenols was assessed using the Folin-Ciocalteu reagent method, while the antioxidant activity of the extracts was evaluated using DPPH and FRAP assays. The polyphenolic compounds were identified by HPLC.
Results. The major polyphenolic compounds identified in the investigated extracts were benzoic acid, vanillic acid, resveratrol and syringic acid. The highest yield of polyphenols ~35.4 mg GAE/g of dried peel was obtained with a solid-to-solvent ratio of 1:20 (w/v) using 20% ultrasonic intensity for 30 min or by treatment with viscozyme at a 1% level for 60 min. The IC50 values by DPPH and FRAP in the ultrasound assisted extract were statistically lower than those in the enzyme assisted extract. The avocado peel extract is a promising source of antioxidants.
Conclusion. The ultrasound assisted extraction proved to be more efficient than enzyme aided extraction in terms of the antioxidant activity of the extractable phenolic compounds.

Keywords: avocado peel, ultrasound, enzyme, extraction, polyphenols, natural antioxidants
pub/.pdf Full text available in english in Adobe Acrobat format:
http://www.food.actapol.net/volume21/issue2/1_2_2022.pdf

https://doi.org/10.17306/J.AFS.2022.0980

For citation:

MLA Hefzalrahman, Tarek, et al. "Application of enzyme and ultrasound assisted extraction of polyphenols from avocado (Persea americana Mill.) peel as natural antioxidants." Acta Sci.Pol. Technol. Aliment. 21.2 (2022): 129-138. https://doi.org/10.17306/J.AFS.2022.0980
APA Hefzalrahman T., Morsi M. K. S., Morsy N. F. S., Hammad K. S. M. (2022). Application of enzyme and ultrasound assisted extraction of polyphenols from avocado (Persea americana Mill.) peel as natural antioxidants. Acta Sci.Pol. Technol. Aliment. 21 (2), 129-138 https://doi.org/10.17306/J.AFS.2022.0980
ISO 690 HEFZALRAHMAN, Tarek, et al. Application of enzyme and ultrasound assisted extraction of polyphenols from avocado (Persea americana Mill.) peel as natural antioxidants. Acta Sci.Pol. Technol. Aliment., 2022, 21.2: 129-138. https://doi.org/10.17306/J.AFS.2022.0980