Acta Scientiarum Polonorum Technologia Alimentaria

ISSN:1644-0730, e-ISSN:1898-9594

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original articleIssue 25 (1) 2026 pp. 117-124

Nguyen Chi Dung1, Nguyen Minh Thuy1, Tran Ngoc Giau1, Hong Van Hao1, Vo Quang Minh2, Ngo Van Tai3

1Institute of Food and Biotechnology, Can Tho University, Vietnam
2
College of Environment and Natural Resources, Can Tho University, Vietnam
3
School of Food Industry, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, Thailand

Functional group identification using FTIR and antho-cyanin profiling by HPLC in purple sweet potato (Ipomoea batatas L.)

Abstract

Background. Purple sweet potato (PSP) has attracted increasing scientific and industrial interest due to its distinctive purple flesh, which is primarily attributed to anthocyanins. Although PSP is produced in large quantities in Vietnam, comprehensive information on its functional groups and anthocyanin pig­ment composition remains limited. Therefore, characterization of functional groups and identification of anthocyanin pigments are essential to support both scientific research and industrial applications of this raw material.
Materials and methods. PSP tubers were collected from farms in Vinh Long Province, Vietnam. The tubers were washed, peeled, sliced, and steam-blanched at 100°C for 3 min, dried at 50°C for 8 h, and milled into powder. Functional groups were characterized using Fourier transform infrared (FTIR) spectroscopy. Antho­cyanins were extracted with acidified methanol and analyzed using high-performance liquid chromatography (HPLC) with external standards for pigment identification and quantification.
Results. FTIR analysis revealed 14 characteristic absorption bands in PSP grown in Vietnam within the wavenumber range of 3288.63–418.55 cm⁻¹. These bands corresponded to hydroxyl, C–H/O–H/N–H, aro­matic (C=O and C=C), ester C–O–C, glycosidic, and pyran ring functional groups, consistent with structural features typical of anthocyanin compounds. HPLC analysis detected three main anthocyanin pigments with retention times of 7.85, 8.77, and 9.93 min, identified as delphinidin, cyanidin, and peonidin, respectively. Their concentrations were 4.034 μg/g (2.2%), 28.490 μg/g (15.5%), and 151.206 μg/g (82.3%).
Conclusion. These results indicate that PSP is a promising source of anthocyanins and has strong potential for use as a functional food with health-promoting properties.

Keywords: anthocyanin pigments; Fourier transform infrared; functional groups; high-performance liquid chromatography (HPLC); purple sweet potato
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https://www.food.actapol.net/volume25/issue1/10_1_2026.pdf

https://doi.org/10.17306/J.AFS.001491

For citation:

MLA Dung, Nguyen Chi, et al. "Functional group identification using FTIR and antho-cyanin profiling by HPLC in purple sweet potato (Ipomoea batatas L.)." Acta Sci.Pol. Technol. Aliment. 25.1 (2026): 117-124. https://doi.org/10.17306/J.AFS.001491
APA Dung N.C., Thuy N.M., Giau T.N., Hao H.V., Minh V.Q., Tai N.V. (2026). Functional group identification using FTIR and antho-cyanin profiling by HPLC in purple sweet potato (Ipomoea batatas L.). Acta Sci.Pol. Technol. Aliment. 25 (1), 117-124 https://doi.org/10.17306/J.AFS.001491
ISO 690 DUNG, Nguyen Chi, et al. Functional group identification using FTIR and antho-cyanin profiling by HPLC in purple sweet potato (Ipomoea batatas L.). Acta Sci.Pol. Technol. Aliment., 2026, 25.1: 117-124. https://doi.org/10.17306/J.AFS.001491