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Issue 8 (1) 2009 pp. 91-97

Patrycja Kłos1, Barbara Poniedziałek2, Eleonora Lampart-Szczapa1, Justyna Gralik1, Krzysztof Wiktorowicz2

1Poznań University of Life Sciences
2
University of Medical Sciences in Poznań

The flow cytometric analysis of lupin proteins’ potential to induce the respiratory burst in human neutrophils

Abstract

There is an ever-increasing demand for lupin seed protein in human nutrition. However, their usage as a food ingredient can be limited by their influence on the immune system. The aim of the study was to analyse, by means of flow cytometry, the potential of lupin seed globulins to induce the respiratory burst in human neutrophils. The results proved the potential of lupin globulins to induce the increased ROS production in human neutrophils. The increased ROS production by the cells treated with lupin globulins and, at the same time, stimulated with the PMA may suggest a synergistic effect of the globulins and the PMA.

Keywords: lupin globulins, immunoreactivity, respiratory burst, reactive oxygen species, neutrophils, phorbol 12-myristate 13-acetate
pub/.pdf Full text available in english in Adobe Acrobat format:
http://www.food.actapol.net/issue1/volume/8_1_2009.pdf

For citation:

MLA Kłos, Patrycja, et al. "The flow cytometric analysis of lupin proteins’ potential to induce the respiratory burst in human neutrophils." Acta Sci.Pol. Technol. Aliment. 8.1 (2009): 91-97.
APA Kłos P., Poniedziałek B., Lampart-Szczapa E., Gralik J., Wiktorowicz K. (2009). The flow cytometric analysis of lupin proteins’ potential to induce the respiratory burst in human neutrophils. Acta Sci.Pol. Technol. Aliment. 8 (1), 91-97
ISO 690 KłOS, Patrycja, et al. The flow cytometric analysis of lupin proteins’ potential to induce the respiratory burst in human neutrophils. Acta Sci.Pol. Technol. Aliment., 2009, 8.1: 91-97.